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dapi staining  (MedChemExpress)


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    MedChemExpress dapi staining
    Dapi Staining, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/DAPI/pm42365506-96-13-24
    Average 98 stars, based on 182 article reviews
    dapi staining - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Formononetin ameliorates IL-13-induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway
    Article Snippet: Subsequently, 5% BSA (cat. no. B265993; Shanghai Aladdin Biochemical Technology Co., Ltd.) diluted in PBS (Beyotime Institute of Biotechnology) was used to block the cells at room temperature for 1 h before incubation with an anti-MUC5AC antibody (1:250) overnight at 4°C. .. Following the primary antibody incubation, the cells were washed with PBS and incubated with a FITC-conjugated goat anti-rabbit IgG secondary antibody (1:200; cat. no. P0186; Beyotime Institute of Biotechnology) at 25°C for 1 h, followed by staining with DAPI (MedChemExpress) for 15 min at room temperature. ..

    Staining:

    Article Title: Formononetin ameliorates IL-13-induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway
    Article Snippet: Subsequently, 5% BSA (cat. no. B265993; Shanghai Aladdin Biochemical Technology Co., Ltd.) diluted in PBS (Beyotime Institute of Biotechnology) was used to block the cells at room temperature for 1 h before incubation with an anti-MUC5AC antibody (1:250) overnight at 4°C. .. Following the primary antibody incubation, the cells were washed with PBS and incubated with a FITC-conjugated goat anti-rabbit IgG secondary antibody (1:200; cat. no. P0186; Beyotime Institute of Biotechnology) at 25°C for 1 h, followed by staining with DAPI (MedChemExpress) for 15 min at room temperature. ..



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    Image Search Results


    Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Journal: Biomaterials and Biosystems

    Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

    doi: 10.1016/j.bbiosy.2026.100137

    Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

    Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

    Techniques: Staining, Cell Culture